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Snap-8 | CAS: Available Upon Request

CAS Number:
Available Upon Request
Chemical Classification:
Research peptide

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Snap-8 is an acetylated octapeptide modeled on enkephalinase-cleavage sites and studied in cosmetic science for its proposed modulation of neuromuscular signaling relevant to expression-line formation. In vitro muscle-contraction and neuronal co-culture systems produce variable magnitude effects depending on peptide concentration and cell-source heterogeneity. Typical experimental setups include organotypic skin models with simulated repetitive contraction, SNARE-protein interaction assays, and comparative testing against longer neurotransmitter-modulating sequences. Research-grade material with analytical documentation; not for commercial cosmetic formulation outside laboratory study.

CAS
Available Upon Request
Molecular Formula
C50H69N15O10
Molecular Weight
1075.2 g/mol
Purity
≥98%
Appearance
White lyophilized powder
Storage
Store at -20°C

Analytical Documentation

COA✓ Available
LC-MS✓ Available
HPLC✓ Available
NMR✓ Available

Research Inquiry

Overview

Snap-8, acetyl glutamyl octapeptide-3, is a synthetic octapeptide extending the hexapeptide argireline motif by two N-terminal glutamyl residues for enhanced aqueous solubility and SNARE complex interaction in cosmetic neuroscience research. Designed as a topical research tool mimicking synaptosome-associated protein SNARE domain interactions, Snap-8 competes with SNAP-25 for inclusion in the SNARE complex in vitro, modulating acetylcholine vesicle fusion in neuronal culture models adapted for dermatological assay development. Snap-8 supports investigation of expression wrinkle formation mechanisms at the neuromuscular junction-skin interface in cosmetic science without botulinum toxin administration. Supplied at ≥98% purity (C50H69N15O10; 1075.2 g/mol), this material supports controlled laboratory investigation under research-use-only conditions.

Mechanism of Action

Snap-8 mimics N-terminal SNAP-25 fragment, binding synaptobrevin and syntaxin with reduced affinity for complete SNARE complex assembly in cell-free fusion assays. Reduced SNARE-mediated exocytosis lowers acetylcholine release at neuromuscular junction models used in cosmetic research platforms. Fibroblast and keratinocyte cultures show indirect effects on muscle contraction mimic systems when paired with motor neuron co-cultures in specialized assays. Mechanism is competitive SNARE interference rather than receptor agonism. Snap-8 does not cleave SNAP-25 unlike botulinum neurotoxin protease activity.

Receptor Binding & Signaling

Snap-8 targets SNARE protein protein-protein interfaces, not GPCRs. No acetylcholine receptor binding occurs; effects are presynaptic fusion inhibition in research models. Off-target protease activity absent in standard biochemical controls.

Research Applications

SNARE complex biochemical assays

Cell-free liposome fusion and SNARE reconstitution experiments add Snap-8 to measure fusion rate reduction versus argireline hexapeptide controls. Syntaxin-SNAP-25 binding assays by surface plasmon resonance validate competitive mechanism. Cosmetic biochemistry laboratories standardize these platforms.

Neuromuscular mimic culture systems

Co-cultures of motor neurons and muscle cell lines or skin equivalents use Snap-8 to quantify contraction-related wrinkle mimic endpoints by imaging in research settings. Acetylcholine ELISA confirms reduced neurotransmitter release. Studies model expression line formation without clinical wrinkle treatment claims.

Comparative argireline analog screening

Structure-activity studies extend hexapeptide to octapeptide variants comparing solubility and SNARE inhibition potency. Snap-8 serves as lead octapeptide in cosmetic peptide discovery research. HPLC purity guides batch selection for assays.

Topical penetration formulation research

Skin models test Snap-8 delivery from cosmetic research vehicles measuring dermal peptide recovery by LC-MS. Penetration enhancers evaluated for assay optimization in supplier and academic cosmetic science collaborations.

Molecular Information

Sequence & Chain Summary

Acetyl-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 (acetyl octapeptide-3).

Modification Type

N-terminal acetylation; C-terminal amide.

Structural Notes

Snap-8 mass approximately 1075.2 g/mol with polar N-terminal Glu-Glu extension improving aqueous solubility over hexapeptide argireline. RP-HPLC confirms ≥98% purity. Acetylation blocks N-terminal charge influencing SNARE mimic binding orientation in structural research. Batch-specific molecular characterization—including mass confirmation and purity profiling—is available through COA, LC-MS, HPLC, and NMR documentation supplied with Snap-8.

Molecular Formula
C50H69N15O10
Molecular Weight
1075.2 g/mol
Purity Specification
≥98%

Experimental Notes

Stability

Lyophilized Snap-8 stable at −20°C desiccated. Reconstituted aqueous solutions stable refrigerated for short cosmetic assay windows. Avoid extreme pH disrupting amide bond over prolonged incubation. Lyophilized Snap-8 should be protected from repeated freeze-thaw cycles, moisture, and prolonged exposure to ambient light where applicable. Analytical integrity is best preserved when material is stored under the conditions specified on the certificate of analysis.

Storage Conditions

Store at -20°C. Snap-8 is supplied as white lyophilized powder. For long-term archival storage in research inventories, maintain sealed containers with desiccant where recommended and document lot numbers for traceability across experimental runs.

Laboratory Handling

Reconstitute in sterile water or assay buffer. Use non-ionic detergents cautiously in SNARE assays where interference possible. Document microgram concentrations per cosmetic research SOP. Reconstitute only with appropriate research-grade solvents compatible with your assay format. Allow vials to reach equilibrium before opening, work under clean bench conditions, and label all working solutions with concentration, date, and researcher ID per institutional SOPs.

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Frequently Asked Questions

Research-focused answers about Snap-8. For laboratory use only — not medical advice.

What is Snap-8 used for in research?
Snap-8 is for cosmetic science research on SNARE-mediated neurotransmitter release models relevant to expression wrinkle biochemistry. Not for injectable neuromodulation or human wrinkle treatment products without regulatory compliance.
How does Snap-8 work biologically?
Snap-8 competes with SNAP-25 in SNARE complex formation, reducing acetylcholine vesicle fusion in research models mimicking presynaptic neuromuscular signaling relevant to cosmetic assay development.
What receptors does Snap-8 interact with?
Targets SNARE protein complexes (SNAP-25 mimic), not cell surface receptors. No acetylcholine receptor engagement; presynaptic fusion mechanism only.
Is Snap-8 stable at room temperature?
Lyophilized Snap-8 stable at −20°C desiccated. Reconstituted aqueous solutions stable refrigerated for short cosmetic assay windows. Avoid extreme pH disrupting amide bond over prolonged incubation. For short-term laboratory workflows, minimize time at room temperature and return unused material to recommended storage promptly. Snap-8 is not formulated for ambient long-term storage.
What is the recommended storage condition for Snap-8?
Store at -20°C. Store lyophilized material in a dedicated −20°C freezer, protect from moisture ingress, and avoid repeated temperature cycling. Reconstituted solutions should be aliquoted and frozen if not used within the validated window of your internal stability study.