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CJC-1295 Without DAC | CAS: 863288-34-0

CAS Number:
863288-34-0
Chemical Classification:
Research peptide

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CJC-1295 Without DAC (CAS 863288-34-0) is a growth hormone-releasing hormone analogue lacking drug-affinity complex modification, producing a shorter plasma exposure profile suited to pulse-timing and receptor-desensitization studies. Without albumin binding, the peptide's pharmacokinetics more closely track bolus injection experiments and short-window tissue sampling. Research questions vary from GHRH-receptor trafficking in recombinant cell lines to pulsatile GH output patterns following discrete subcutaneous administration. Bench use often involves timed serum GH immunoassays, pituitary slice electrophysiology, and head-to-head comparison with DAC-modified analogs under matched molar dosing. Analytical documentation includes COA, LC-MS, HPLC, and NMR; laboratory research use only.

CAS
863288-34-0
Molecular Formula
C152H252N44O42
Molecular Weight
3367.9 g/mol
Purity
≥98%
Appearance
White lyophilized powder
Storage
Store at -20°C

Analytical Documentation

COA✓ Available
LC-MS✓ Available
HPLC✓ Available
NMR✓ Available

Research Inquiry

Overview

CJC-1295 without DAC is a synthetic 29-amino-acid peptide analog corresponding to the biologically active fragment of growth hormone-releasing hormone, modified at four positions to enhance enzymatic stability relative to native GHRH(1-29). The designation without DAC indicates absence of the maleimidopropionyl drug affinity complex that enables albumin conjugation in the long-acting CJC-1295 variant, resulting in shorter plasma exposure suitable for acute secretagogue experiments. The peptide activates GHRH receptor on pituitary somatotrophs, providing a research tool for pulsatile GH stimulation studies without sustained albumin-linked release. CJC-1295 without DAC is widely used in endocrine research comparing GHRH-mimetic pathways with ghrelin receptor agonists and investigating somatotroph desensitization kinetics. Supplied at ≥98% purity (C152H252N44O42; 3367.9 g/mol), this material supports controlled laboratory investigation under research-use-only conditions.

Mechanism of Action

CJC-1295 without DAC binds GHRH receptor and stimulates Gs-adenylyl cyclase signaling, increasing intracellular cAMP and promoting acute GH vesicle exocytosis from somatotrophs. Substitutions at positions 2, 8, 15, and 27 confer resistance to DPP-IV and other peptidases, extending activity in plasma-containing assay media compared with unmodified GHRH(1-29). Without DAC modification, the peptide lacks covalent albumin anchoring, producing sharper GH peaks in research animals following bolus administration. Chronic infusion studies reveal GHRH receptor desensitization patterns distinct from ghrelin pathway activation. The compound does not act at GHS-R1a at research-relevant concentrations.

Receptor Binding & Signaling

Primary target is GHRH receptor on anterior pituitary somatotrophs with high affinity and full agonism in cAMP assays. Selectivity over GHS-R1a, GLP-1R, and unrelated class B receptors is maintained at standard research doses. Human versus rodent GHRHR sequence differences modulate EC50 values in cross-species translation studies.

Research Applications

Acute GHRH receptor stimulation

Bolus administration in rodent research produces defined GH peaks for pulse deconvolution analysis without prolonged elevation associated with albumin-conjugated analogs. Pituitary perifusion systems compare CJC-1295 without DAC with native GHRH fragments. These protocols map acute secretagogue kinetics in endocrine physiology research.

Somatotroph desensitization studies

Repeated dosing paradigms examine GHRH receptor downregulation, internalization, and GH response attenuation over multi-day research schedules. Pairing with somatostatin measurements clarifies feedback adaptation. CJC-1295 without DAC provides controllable exposure windows for desensitization modeling.

Comparative secretagogue profiling

Factorial experiments contrast GHRH analogs with ipamorelin and other GHS-R ligands on GH, prolactin, and cortisol release in research animals. CJC-1295 without DAC represents the GHRH arm with defined pharmacokinetics. Data support pathway-resolved endocrine research.

In vitro GHRHR pharmacology

Recombinant cells expressing GHRHR validate peptide potency via cAMP and calcium imaging after CJC-1295 without DAC stimulation. Batch QC relies on EC50 reporting against reference standards. Antagonist blockade confirms target specificity in assay development.

Molecular Information

Sequence & Chain Summary

Modified GHRH(1-29): D-Ala2, Gln8, Ala15, Leu27 substitutions.

Modification Type

No Drug Affinity Complex (DAC); tetrasubstituted GHRH(1-29) scaffold.

Structural Notes

The 29-amino-acid peptide maintains alpha-helical structure for GHRH receptor engagement confirmed by mass spectrometry and HPLC purity profiling. Absence of DAC eliminates maleimide linker chemistry present in long-acting variants. SPPS with side-chain protection yields product requiring lyophilization and moisture-protected storage. Batch-specific molecular characterization—including mass confirmation and purity profiling—is available through COA, LC-MS, HPLC, and NMR documentation supplied with CJC-1295 Without DAC.

Molecular Formula
C152H252N44O42
Molecular Weight
3367.9 g/mol
Purity Specification
≥98%

Experimental Notes

Stability

Lyophilized peptide stable at −20°C. Without albumin binding, reconstituted solutions degrade faster at room temperature than DAC-conjugated forms; use fresh aliquots when possible. Minimize freeze-thaw cycles. DPP-IV resistance improves stability in serum-containing media during short incubations. Lyophilized CJC-1295 Without DAC should be protected from repeated freeze-thaw cycles, moisture, and prolonged exposure to ambient light where applicable. Analytical integrity is best preserved when material is stored under the conditions specified on the certificate of analysis.

Storage Conditions

Store at -20°C. CJC-1295 Without DAC is supplied as white lyophilized powder. For long-term archival storage in research inventories, maintain sealed containers with desiccant where recommended and document lot numbers for traceability across experimental runs.

Laboratory Handling

Reconstitute with bacteriostatic or sterile water. Administer in research models promptly after preparation when studying acute GH dynamics. Store unused aliquots frozen. Verify concentration by HPLC or UV when available. Reconstitute only with appropriate research-grade solvents compatible with your assay format. Allow vials to reach equilibrium before opening, work under clean bench conditions, and label all working solutions with concentration, date, and researcher ID per institutional SOPs.

Related Peptides

Growth Hormone Peptides →Peptide CAS database →

Frequently Asked Questions

Research-focused answers about CJC-1295 Without DAC. For laboratory use only — not medical advice.

What is CJC-1295 Without DAC used for in research?
CJC-1295 without DAC is for laboratory research on GHRH receptor-mediated growth hormone release and somatotroph physiology. Not for human or veterinary use or commercial compounding.
How does CJC-1295 Without DAC work biologically?
This GHRH analog activates pituitary GHRH receptors to increase cAMP and trigger acute GH secretion in research models. Lack of DAC modification yields shorter activity profile than albumin-conjugated CJC-1295 variants.
What receptors does CJC-1295 Without DAC interact with?
Primary target is GHRH receptor (GHRHR) on somatotrophs. Minimal ghrelin receptor engagement distinguishes it from GHS-R agonists in standard endocrine research assays.
Is CJC-1295 Without DAC stable at room temperature?
Lyophilized peptide stable at −20°C. Without albumin binding, reconstituted solutions degrade faster at room temperature than DAC-conjugated forms; use fresh aliquots when possible. Minimize freeze-thaw cycles. DPP-IV resistance improves stability in serum-containing media during short incubations. For short-term laboratory workflows, minimize time at room temperature and return unused material to recommended storage promptly. CJC-1295 Without DAC is not formulated for ambient long-term storage.
What is the recommended storage condition for CJC-1295 Without DAC?
Store at -20°C. Store lyophilized material in a dedicated −20°C freezer, protect from moisture ingress, and avoid repeated temperature cycling. Reconstituted solutions should be aliquoted and frozen if not used within the validated window of your internal stability study.