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Cagrilintide | CAS: 1415456-99-3

CAS Number:
1415456-99-3
Chemical Classification:
Research peptide

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Cagrilintide is a long-acting amylin receptor agonist analogue (CAS 1415456-99-3) explored alongside incretin peptides when satiety and postprandial hormone integration are central to the experimental design. Its fatty-acid conjugation extends receptor engagement relative to native amylin, giving pharmacokinetic flexibility that single-injection rodent paradigms often require. The relevance of amylin-pathway readouts shifts with model selection—pair-feeding studies emphasize behavioral components, whereas portal hormone sampling stresses endocrine coordination. Researchers frequently combine the analogue with GLP-1R ligands in co-administration protocols, run hypothalamic neuropeptide expression panels after acute dosing, and quantify gastric-retention kinetics using non-absorbed tracers. Batches are released with COA, LC-MS, HPLC, and NMR data for laboratory use only.

CAS
1415456-99-3
Molecular Formula
C194H295N45O59
Molecular Weight
4258.7 g/mol
Purity
≥98%
Appearance
White lyophilized powder
Storage
Store at -20°C

Analytical Documentation

COA✓ Available
LC-MS✓ Available
HPLC✓ Available
NMR✓ Available

Research Inquiry

Overview

Cagrilintide is a long-acting synthetic analog of amylin, the 37-amino-acid peptide co-secreted with insulin from pancreatic beta cells. Engineered with amino acid substitutions and acylation that confer protease resistance and extended half-life, cagrilintide acts as an amylin receptor agonist in metabolic research without relying on native peptide instability. Amylin participates in postprandial glycemic control by slowing gastric emptying, suppressing inappropriate glucagon secretion, and promoting satiety through central and peripheral pathways. Cagrilintide enables investigators to model amylin receptor pharmacology alongside GLP-1 pathway tools, dissecting how amylin signaling complements incretin biology in islet-periphery communication and energy intake regulation in controlled animal and cell-based systems. Supplied at ≥98% purity (C194H295N45O59; 4258.7 g/mol), this material supports controlled laboratory investigation under research-use-only conditions.

Mechanism of Action

Cagrilintide activates amylin receptors formed by calcitonin receptor (CTR) and receptor activity-modifying proteins (RAMP1, RAMP2, or RAMP3), predominantly the AMY1R (CTR/RAMP1) complex in research-relevant tissues. Gs and Gq coupling elevates cAMP and intracellular calcium, modulating vagal tone that slows gastric emptying and reduces postprandial glucagon peaks in rodent models. Central amylin receptor populations in the area postrema and hypothalamus contribute to anorectic signaling when the peptide accesses circumventricular organs in research dosing. Cagrilintide does not directly stimulate insulin secretion but alters nutrient appearance and alpha-cell activity indirectly. Combination research with GLP-1 agonists explores synergistic satiety and glycemic endpoints in preclinical metabolic platforms.

Receptor Binding & Signaling

Cagrilintide binds amylin receptor complexes with high affinity, especially AMY1R and AMY3R configurations depending on tissue expression profile. Native amylin and salmon calcitonin serve as comparative ligands in competition binding studies. Selectivity over calcitonin receptor homodimers and unrelated class B receptors is maintained at research concentrations through RAMP-dependent pharmacology. Acylation extends plasma residence, altering receptor occupancy kinetics in pharmacokinetic experiments. Species-specific RAMP expression patterns influence translation between human cell assays and rodent in vivo models.

Research Applications

Amylin receptor signaling assays

Cell lines co-expressing CTR and RAMP subunits are stimulated with cagrilintide to measure cAMP, ERK phosphorylation, and internalization relative to native amylin. These platforms validate batch potency and support structure-activity studies on acylation position and amino acid substitutions. Antagonists such as AC187 help confirm receptor-specific responses. Cagrilintide serves as a stable reference ligand replacing native amylin in long-duration incubations. Results inform amylin receptor pharmacology without extrapolation to clinical nutrition research.

Glucagon suppression and alpha-cell biology

Islet perfusion and alpha-cell sorted preparations use cagrilintide to evaluate suppression of glucagon secretion during hyperglycemic and euglycemic clamps in research animals. Investigators measure pulsatile glucagon patterns and paracrine crosstalk with beta cells. Amylin receptor knockdown models attribute effects to specific receptor complexes. Pairing with GLP-1 agonists tests additive mechanisms on alpha-cell output. These experiments advance understanding of islet hormone coordination in metabolic research.

Satiety and intake behavior models

Rodent feeding paradigms including cumulative intake, meal pattern analysis, and conditioned taste aversion use cagrilintide to probe amylin-driven anorectic signaling. Central versus peripheral administration routes distinguish area postrema from hypothalamic mechanisms. Cagrilintide supports comparison with selective GLP-1 agonists to map non-overlapping satiety pathways. Behavioral studies remain within preclinical research ethics frameworks. Data contribute to neuroendocrine models of energy intake regulation.

Combination incretin-amylin research

Laboratory protocols co-administer cagrilintide with GLP-1 receptor agonists to study synergistic effects on gastric emptying, glycemic excursions, and body composition endpoints in rodent models. Factorial designs separate amylin-only, GLP-1-only, and combined arms with appropriate controls. Cagrilintide enables stable amylin receptor engagement over multi-week study durations. Researchers document receptor occupancy hypotheses alongside phenotypic readouts. These combination studies are confined to basic metabolic science.

Molecular Information

Sequence & Chain Summary

Amylin sequence analog with amino acid substitutions at positions 25, 28, and 29 (proline-rich region modified).

Modification Type

C20 fatty di-acid acylation for extended half-life.

Structural Notes

Cagrilintide retains the disulfide-linked helical structure characteristic of amylin and calcitonin family peptides, requiring careful handling to preserve oxidative folding integrity during synthesis QC. Mass spectrometry confirms acyl adduct and disulfide connectivity. RP-HPLC resolves oxidized and reduced forms when present as impurities. The peptide's hydrophobic acyl chain increases lipophilicity, affecting solubilization protocols in aqueous research buffers. Batch-specific molecular characterization—including mass confirmation and purity profiling—is available through COA, LC-MS, HPLC, and NMR documentation supplied with Cagrilintide.

Molecular Formula
C194H295N45O59
Molecular Weight
4258.7 g/mol
Purity Specification
≥98%

Experimental Notes

Stability

Lyophilized cagrilintide is stable at −20°C when sealed and desiccated. Reconstituted solutions should be used promptly or aliquoted at −80°C for extended storage. Protect from repeated freeze-thaw cycles that may reduce amylin receptor activity. Avoid alkaline conditions that could disrupt disulfide bonds during prolonged incubation. Lyophilized Cagrilintide should be protected from repeated freeze-thaw cycles, moisture, and prolonged exposure to ambient light where applicable. Analytical integrity is best preserved when material is stored under the conditions specified on the certificate of analysis.

Storage Conditions

Store at -20°C. Cagrilintide is supplied as white lyophilized powder. For long-term archival storage in research inventories, maintain sealed containers with desiccant where recommended and document lot numbers for traceability across experimental runs.

Laboratory Handling

Reconstitute with mild acidified water or buffer if solubility is limited due to fatty acylation. Use non-stick labware for dilute stocks. Verify peptide integrity by HPLC before long experiments. Store working solutions protected from light and microbial contamination according to SOP. Reconstitute only with appropriate research-grade solvents compatible with your assay format. Allow vials to reach equilibrium before opening, work under clean bench conditions, and label all working solutions with concentration, date, and researcher ID per institutional SOPs.

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Frequently Asked Questions

Research-focused answers about Cagrilintide. For laboratory use only — not medical advice.

What is Cagrilintide used for in research?
Cagrilintide is provided for research on amylin receptor biology, islet hormone interplay, and metabolic neuroendocrine signaling in laboratory settings. It is not for human or veterinary administration or use in food products. Follow institutional guidelines for peptide handling and animal research where applicable.
How does Cagrilintide work biologically?
Cagrilintide activates amylin receptor complexes to slow gastric emptying, reduce glucagon secretion, and promote satiety signaling in experimental models. Its long-acting design supports sustained receptor engagement in pharmacology studies compared with native amylin.
What receptors does Cagrilintide interact with?
Primary targets are amylin receptors (AMY1R, AMY3R) comprising calcitonin receptor plus RAMP co-receptors. Cross-reactivity with calcitonin receptor homodimers is lower than for native calcitonin at equivalent research doses.
Is Cagrilintide stable at room temperature?
Lyophilized cagrilintide is stable at −20°C when sealed and desiccated. Reconstituted solutions should be used promptly or aliquoted at −80°C for extended storage. Protect from repeated freeze-thaw cycles that may reduce amylin receptor activity. Avoid alkaline conditions that could disrupt disulfide bonds during prolonged incubation. For short-term laboratory workflows, minimize time at room temperature and return unused material to recommended storage promptly. Cagrilintide is not formulated for ambient long-term storage.
What is the recommended storage condition for Cagrilintide?
Store at -20°C. Store lyophilized material in a dedicated −20°C freezer, protect from moisture ingress, and avoid repeated temperature cycling. Reconstituted solutions should be aliquoted and frozen if not used within the validated window of your internal stability study.