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BPC-157 (Acetate) | CAS: 137525-51-0

CAS Number:
137525-51-0
Chemical Classification:
Research peptide

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BPC-157 (Acetate) (CAS 137525-51-0) is a synthetic pentadecapeptide derived from gastric juice protein fragments, widely adopted in regenerative and tissue-repair research for its reported effects on angiogenic and cytoprotective pathways. Outcomes are sensitive to injury model, route of administration, and species, so cross-study comparisons require careful alignment of dosing schedules and histological endpoints. Laboratories apply the acetate salt in tendon transection models, dermal wound-closure time courses, and endothelial migration assays with distinct emphasis on vascular versus matrix remodeling. Standard preparations are characterized by COA, LC-MS, HPLC, and NMR; not intended for human or veterinary therapeutic use.

CAS
137525-51-0
Molecular Formula
C62H98N16O22
Molecular Weight
1419.5 g/mol
Purity
≥98%
Appearance
White lyophilized powder
Storage
Store at -20°C

Analytical Documentation

COA✓ Available
LC-MS✓ Available
HPLC✓ Available
NMR✓ Available

Research Inquiry

Overview

BPC-157 is a synthetic pentadecapeptide corresponding to a fragment of body protection compound derived from human gastric juice, specifically a stable region of a larger protein identified in gastric secretions. The sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val has attracted research interest for its effects on angiogenesis, nitric oxide pathways, and growth factor signaling in tissue culture and animal models of mechanical injury. Supplied as the acetate salt, BPC-157 serves as a pharmacological probe in regenerative biology research examining extracellular matrix remodeling, endothelial cell migration, and tendon fibroblast proliferation under controlled laboratory conditions. Its small size and linear structure distinguish it from larger thymosin-derived peptides studied in parallel wound-healing research platforms. Supplied at ≥98% purity (C62H98N16O22; 1419.5 g/mol), this material supports controlled laboratory investigation under research-use-only conditions.

Mechanism of Action

BPC-157 modulates nitric oxide synthase activity and VEGF receptor 2 expression in endothelial and fibroblast research models, promoting angiogenic tube formation in Matrigel assays without direct growth factor receptor agonism at canonical binding sites. The peptide interacts with dopamine D2 receptor and serotonin 2a receptor in some binding studies, suggesting neuromodulatory cross-talk that may influence local blood flow in injury models. FAK-paxillin pathway activation supports fibroblast spreading and collagen organization in tendon explant research. BPC-157 upregulates growth hormone receptor expression in some musculoskeletal cell lines, linking local repair phenotypes to endocrine signaling in exploratory experiments. Mechanisms remain multifactorial and context-dependent across tissue types studied in basic science.

Receptor Binding & Signaling

Direct high-affinity binding to a single canonical receptor remains unresolved; research reports interactions with VEGFR2 signaling complexes, dopamine D2 receptor, and 5-HT2a receptor in radioligand and Western blot co-localization studies. BPC-157 does not activate GH receptor directly. Effects on eNOS and iNOS isoforms occur at transcriptional and post-translational levels in cell culture. Off-target GPCR screening at research concentrations shows limited promiscuity beyond reported neuromodulatory sites.

Research Applications

Angiogenesis and endothelial migration

Human umbilical vein endothelial cells and microvascular endothelial models use BPC-157 to quantify scratch wound closure, tube formation, and VEGFR2 phosphorylation in serum-reduced conditions. Antagonists and NOS inhibitors test pathway dependency. These assays advance vascular biology research without clinical wound-healing claims.

Tendon and ligament fibroblast studies

Primary tenocyte cultures and tendon explants expose tissue to BPC-157 to measure collagen I synthesis, cell proliferation, and FAK activation in mechanical load or cytokine challenge models. Rodent tendon transection research documents histological and biomechanical endpoints under controlled protocols. Studies focus on musculoskeletal regenerative biology in preclinical settings.

Gastrointestinal mucosal research

Given its gastric origin, BPC-157 is applied in rodent models of chemically induced mucosal injury to study re-epithelialization, submucosal angiogenesis, and inflammatory mediator profiles. Endoscopic and histopathological scoring provides structured readouts. Research explores local protective signaling pathways in GI tract biology laboratories.

Nitric oxide and vascular tone models

Isolated vessel myography and NOS expression studies examine whether BPC-157 modifies vasodilation and endothelial function in research animals. L-NAME and selective NOS isoform tools deconvolute mechanism. BPC-157 supports cardiovascular physiology research linked to angiogenic peptide biology.

Molecular Information

Sequence & Chain Summary

Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (15 amino acids).

Modification Type

Acetate salt form.

Structural Notes

BPC-157 is a linear peptide without disulfide bonds, simplifying synthesis and HPLC purification. Proline-rich segments influence conformational flexibility and may affect protease susceptibility. Mass confirmation by ESI-MS aligns with expected 1419.5 g/mol free base. Purity ≥98% verified by RP-HPLC. Batch-specific molecular characterization—including mass confirmation and purity profiling—is available through COA, LC-MS, HPLC, and NMR documentation supplied with BPC-157 (Acetate).

Molecular Formula
C62H98N16O22
Molecular Weight
1419.5 g/mol
Purity Specification
≥98%

Experimental Notes

Stability

Lyophilized acetate salt stable at −20°C desiccated. Reconstituted aqueous solutions tolerate short bench exposure; refrigerate for same-day use. Proline-rich peptides resist some serine proteases but degrade in strong alkaline conditions. Avoid repeated freeze-thaw of working stocks. Lyophilized BPC-157 (Acetate) should be protected from repeated freeze-thaw cycles, moisture, and prolonged exposure to ambient light where applicable. Analytical integrity is best preserved when material is stored under the conditions specified on the certificate of analysis.

Storage Conditions

Store at -20°C. BPC-157 (Acetate) is supplied as white lyophilized powder. For long-term archival storage in research inventories, maintain sealed containers with desiccant where recommended and document lot numbers for traceability across experimental runs.

Laboratory Handling

Reconstitute in sterile water or PBS for cell culture compatibility. Filter sterilize if required for sensitive assays. Document mg/mL and mM concentrations. Use clean technique to prevent microbial contamination in long incubations. Reconstitute only with appropriate research-grade solvents compatible with your assay format. Allow vials to reach equilibrium before opening, work under clean bench conditions, and label all working solutions with concentration, date, and researcher ID per institutional SOPs.

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Frequently Asked Questions

Research-focused answers about BPC-157 (Acetate). For laboratory use only — not medical advice.

What is BPC-157 (Acetate) used for in research?
BPC-157 acetate is for regenerative biology and tissue culture research investigating angiogenic and fibroblast signaling pathways. Not for human or veterinary treatment or cosmetic application on humans.
How does BPC-157 (Acetate) work biologically?
BPC-157 modulates VEGF signaling, nitric oxide pathways, and fibroblast migration in research models through multifactorial mechanisms not fully attributed to a single receptor. Effects vary by tissue context in preclinical studies.
What receptors does BPC-157 (Acetate) interact with?
No single primary receptor is established; research implicates VEGFR2 signaling modulation, dopamine D2, and 5-HT2a interactions depending on experimental system. GH receptor is not a direct target.
Is BPC-157 (Acetate) stable at room temperature?
Lyophilized acetate salt stable at −20°C desiccated. Reconstituted aqueous solutions tolerate short bench exposure; refrigerate for same-day use. Proline-rich peptides resist some serine proteases but degrade in strong alkaline conditions. Avoid repeated freeze-thaw of working stocks. For short-term laboratory workflows, minimize time at room temperature and return unused material to recommended storage promptly. BPC-157 (Acetate) is not formulated for ambient long-term storage.
What is the recommended storage condition for BPC-157 (Acetate)?
Store at -20°C. Store lyophilized material in a dedicated −20°C freezer, protect from moisture ingress, and avoid repeated temperature cycling. Reconstituted solutions should be aliquoted and frozen if not used within the validated window of your internal stability study.